![]() Validation of array comparative genome hybridization for diagnosis of translocations in preimplantation human embryos. 2014 20(9):499–508.Ĭolls P, Escudero T, Fischer J, Cekleniak NA, Ben-Ozer S, Meyer B, et al. Embryonic aneuploidy: overcoming molecular genetics challenges improves outcomes and changes practice patterns. First births after preimplantation genetic diagnosis of structural chromosome abnormalities using comparative genomic hybridization and microarray analysis. PGD for reciprocal and Robertsonian translocations using array comparative genomic hybridization. 2016 22(8):845–57.įiorentino F, Spizzichino L, Bono S, Biricik A, Kokkali G, Rienzi L, et al. The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists. Sermon K, Capalbo A, Cohen J, Coonen E, De Rycke M, De Vos A, et al. Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study. Yang Z, Liu J, Collins GS, Salem SA, Liu X, Lyle SS, et al. Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos. Gutierrez-Mateo C, Colls P, Sanchez-Garcia J, Escudero T, Prates R, Ketterson K, et al. Why do euploid embryos miscarry? A case-control study comparing the rate of aneuploidy within presumed euploid embryos that resulted in miscarriage or live birth using next-generation sequencing. Maxwell SM, Colls P, Hodes-Wertz B, DH MC, McCaffrey C, Wells D, et al. Introduction: to transfer or not transfera mosaic embryo, that is the question. Mosaicism in health and disease-clones picking up speed. Evidence for high frequency of chromosomal mosaicism in spontaneous abortions revealed by interphase FISH analysis. Vorsanova SG, Kolotii AD, Iourov IY, Monakhov VV, Kirillova EA, Soloviev IV, et al. ![]() High rate of mixoploidy among human blastocysts cultured in vitro. FISH reanalysis of inner cell mass and trophectoderm samples of previously array-CGH screened blastocysts shows high accuracy of diagnosis and no major diagnostic impact of mosaicism at the blastocyst stage. 2000 15(8):1781–6.Ĭapalbo A, Wright G, Elliott T, Ubaldi FM, Rienzi L, Nagy ZP. Chromosome mosaicism in day 3 aneuploid embryos that develop to morphologically normal blastocysts in vitro. Magli MC, Jones GM, Gras L, Gianaroli L, Korman I, Trounson AO. ![]() Mosaicism in the inner cell mass of human blastocysts. Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients. Mosaicism in preimplantation human embryos: when chromosomal abnormalities are the norm. Detection of aneuploidy and chromosomal mosaicism in human embryos during preimplantation sex determination by fluorescent in situ hybridisation (FISH). 2014 20(4):571–81.ĭelhanty JD, Griffin DK, Handyside AH, Harper J, Atkinson GH, Pieters MH, et al. The origin, mechanisms, incidence and clinical consequences of chromosomal mosaicism in humans. Taylor TH, Gitlin SA, Patrick JL, Crain JL, Wilson JM, Griffin DK. However, as mosaic embryo transfer yielded a live birth rate of 46.6%, patients without euploid embryos could be counseled regarding this alternative option. ConclusionsĬompared with euploid embryo transfer, mosaic embryo transfer is associated with a lower rate of live birth, which is mainly attributed to a decreased rate of conception. The rates of clinical pregnancy and pregnancy loss and the risks of obstetric outcomes did not differ significantly between the two groups. 76.1%, OR 0.60, 95% CI 0.37–0.99) per transfer cycle were significantly lower after mosaic embryo transfer than after euploid embryo transfer. Additionally, 268 euploid embryos were matched as controls. Overall, 102 embryos were determined to be mosaic, of which 101 were estimated to harbor no more than 50% aneuploid mosaicism. Mosaic and control euploid embryos were defined according to log2 ratio calls. The original aCGH charts of the transferred embryos were reanalyzed. The embryos, which had been transferred as “euploidy,” were processed using array-based comparative genomic hybridization (aCGH). This retrospective cohort study aimed to investigate pregnancy outcomes after transfer of mosaic or euploid embryos. ![]() Mosaicism is a prevalent characteristic of human preimplantation embryos. ![]()
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